Choosing and preparing culture media (UPDATE 07/03/2020)

 

Before preparing your environment, you will already determine that you need according to your crops. You will find below a summary that lets you choose:



Epithytic Orchid 

Genus
Germination
Replate
Ascocenda K400, p668, p723 p668
Brassia K400, p723 K400 + coco powder
Cattleya K400 + coco powder, p668, p723, KOFF + K400 + coco powder, 1/2 p668, KOFF MEDIA6, KOFF +
Cyrtopodium p723 723, KOFF MEDIA6
Dendrobium K400 + coco powder, M579 M579, K400 + coco powder
Dendrophylax p723 p723, KOFF MEDIA6
Encyclia p723, KOFF MEDIA6 KOFF MEDIA6
Epidendrum K400 + coco powder,  p668, p723, M579 K400 + coco powder, p668, KOFF MEDIA6
Gongora K400 + coco powder, p723 K400 + coco powder, KOFF MEDIA6
Maxillaria
p723 KOFF MEDIA6
Oncidium
 p668, p723 p688, p723, KOFF MEDIA6
Odontoglossum
K400 + coco powder, p723 K400
Phalaenopsis
K400, KOFF MEDIA1, p668, p723 KOFF MEDIA1, p668, KOFF MEDIA6
Pleurothallis
p668, p723 KOFF MEDIA6
Prosthechea
p723 p723, KOFF MEDIA6
Schomburgkia
p723 p723, KOFF MEDIA6
Vanda
K400, P723, M579 p723, KOFF MEDIA6, M579
Vanille
K400 + coco powder K400 + coco powder

Terrestrial orchid

genus
Germination
Replate
Aplectrum
K400 K400 + banana powder
Arethusa
K400 + P723 K400 + banana powder, KOFF MEDIA6
Bletia
K400, P723,B141 KOFF MEDIA6
Bletilla
P668 1/2, P723 P668, KOFF MEDIA6
Calopogon
K400, P723 P723, KOFF MEDIA6
Calypso
F522,B141, B142 F522
Coeloglossum
K400 K400 + banana powder, KOFF MEDIA6
Cymbidium
M579 + 2g petone (P775) M579
Cypripedium

B141 + BA, F522, K400 1/2 + poudre de coco

M534, B142

B141 + BA, F522, K400 1/2 + banana powder, P668 1/2
Dactylorhiza
F522 F522, KOFF MEDIA6
Epipactis
B141 + BA, B142 B141 + BA, P668
Eulophia
P723 KOFF MEDIA6
Goodyera
P668 1/2, P723,M534 KOFF MEDIA6
Gymnadenia
F522, P668 1/2,M534 F522, KOFF MEDIA6,M534
Limodorum
B141 + BA, B142 B141 + BA, B142
Liparis
K400 K400 + banana powder, KOFF MEDIA6
Oeceoclades
P668 1/2, P723 P723
Ophrys
M534, P668 1/2 M534
Orchis M53, F522, P668 1/2 M534, F522
Paphiopedilum
P668 1/2 P668 1/2,
Phragmipedium
P668 1/2 P668 1/2,
Platanthera
K400, F522, M534, P668 1/2, P723 F522, M534, P723, KOFF MEDIA6
Pogonia
K400 K400
Habenaria
M534 + P723 M534 + P723
Sacoila
K400 + M534
Spathoglottis
P723 P668, P723, KOFF MEDIA6
Spiranthes
K400, M534, P723 M534, KOFF MEDIA6
Thelymitra
P723 KOFF MEDIA6
Tipularia
K400 K400 + banana powder
Zeuxine
P723 P723 , KOFF MEDIA6

Some require the addition of agar (7 g per 1 literto verify the composition of the product.

Once your selected culture medium, on to preparing it.

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Preparation of culture medium

1. Preparing the media:

Generally prepared culture medium one week in advance, to check that containers have not been contaminated during preparationThe preparation is the same, regardless of the chosen medium.
It's like a recipe:
a) In a large pot, pour 1 liter of distilled water, deionized water or osmosis waterHeat until the water boilsThen turn down the heatto stop the boil but keep good hot water (simmering)
b) Caution: If the medium already contains agar, skip to step c.
Agar pour into the pan and stir with a whisk periodically until the mixture again become clear (approximately 10 minutes). It takes a dose of 7 grams of agar for 1L culture medium.

c) Pour carefully the media bag in the pan, because the powder is very volatile and could spread easily in the event of inadequate gesture.
d) Allow to heat without boiling, whisking regularly for 10 to 15 minutes at least.
e) Remove from heat and pour the medium into your tuperware, about 1cm thick per container (with a syringe, it's easier)
f) Drill the covers of a 1mm hole diameter and place a micropore tape piece. Put the lid when completely cooled.

  

     etape 4.jpg

2. Sterilization of tuperwares:

a) tuperwares are placed in the basket steam pressure cooker in which one has previously paid a water depth of approximately 1 cm.
b) Close the pressure cooker and put it there on high heat
c) When the pressure cooker starts to whistlelower the heat so that the valve whispers gently and count then 24 minutes 'cooking'
d) The 20 minutes are upturn off the heat (or the casserole out of the plate if your stove is electricfall completely and let the pressure cooker and cool completely before opening

e) Take them out of the pressure cookerOn cooling, the agar solidifies. Shoot them with cellophane so as not to allow air to enterThey can be twisted out of the pressure cooker but will resume their original shape after cooling.
After two hours, store your containers at room temperature, protected from shocks (buffet, shelves, etc.) can also be stored in the refrigerator if you do not wish to use them for several weeks.
f) After three or four days, check them for possible contamination. Discard those that you consider questionable (whitish deposit, moss, etc. ...)

Exemple de contamination


 

Sterilization of Microbox

 
 
Sterilization of these boxes will be with a wick placed in a corner and closed lid the other 3 corners. Place the boxes and start the fire so that the pressure rises slowly.

After 25 minutes of cooking, let the pressure down and Ouveze your casserole.

Remove the strip and close the last corner.

 

 
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